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M94B0779.TXT
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1994-11-11
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Document 0779
DOCN M94B0779
TI Subtypes of Epstein-Barr virus in HIV-1-positive and HIV-1-negative
patients with Hodgkin's disease (Meeting abstract).
DT 9412
AU De Re V; De Vita S; Marzotto A; Gloghini A; Carbone A; Uccini S; Scarpa
A; and Boiocchi M; Div. of Experimental Oncology, Aviano, Italy
SO EACR-12: 12th Biennial Meeting of the European Association for Cancer
Research. April 4-7, 1993, Brussels, Belgium, 1993.. Unique Identifier :
AIDSLINE ICDB/94697510
AB Considerable evidence suggests that EBV has a role in the pathogenesis
of Hodgkin's disease (HD). In immunocompetent hosts type 2 EBV is
considered to be a much less potent transformer of lymphocytes than type
1 virus strain. However, type 2 EBV may be involved in the pathogenesis
of non-Hodgkin's lymphomas arising in immunocompromised patients (ie,
those with HIV-1 or malarial infection). To determine whether type 2 EBV
may also play a role in similar patients developing HD, we characterized
EBV subtypes, using the PCR technology, in 36 tumor samples (previously
demonstrated to be EBV-positive by amplification of the IR3 genome
sequence common to both EBV strains) from 10 HIV-1-positive patients as
well as from a control population of 26 HIV-1-negative patients. Type 2
EBV was detected in 5 samples from HIV-1-positive patients, and in only
1 sample from the HIV-1-negative group. DNA of one HIV-1-positive
patient and DNA of one HIV-1-negative patient was positive for both EBV
type 1 and EBV type 2. DNA sequence analysis of EBNA-2 region indicated
the concomitant presence of both EBV type 1 and EBV type 2 expansions
and excluded a possible 'hybrid-recombinant' in these two tumor samples.
A biclonal EBV tumor infection was also detected by Southern blot in two
additional HIV-1-negative cases, both clones belonging, this time, to
type 1 virus strain. In six EBV-positive cases (all from HIV-1-negative
patients), characterization of the virus could not be performed
presumably on account of deletion including EBNA-2 region. Our results
indicate that in HIV-1-infected patients type 2 EBV presents a
transforming ability that is roughly similar to that shown by type 1
virus strain also in patients developing HD.
DE Acquired Immunodeficiency Syndrome/COMPLICATIONS/*MICROBIOLOGY
Antigens, Viral/GENETICS DNA-Binding Proteins/GENETICS Gene
Amplification Genome, Viral HIV Seropositivity/GENETICS/*MICROBIOLOGY
*Herpesvirus 4, Human/GENETICS Hodgkin's
Disease/COMPLICATIONS/GENETICS/*MICROBIOLOGY Human MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).